Automated Parallel Recordings of Topologically Identified Single Ion Channels

摘要: Although ion channels are attractive targets for drug discovery, the systematic screening of channel-targeted drugs remains challenging. To facilitate automated single ion-channel recordings analysis interactions with intra- and extracellular domain, we have developed a parallel recording methodology using artificial cell membranes. The use stable lipid bilayer formation in droplet chamber arrays facilitated automated, parallel, single-channel from reconstituted native mutated channels. Using this system, several types channels, including forms, were characterised by determining protein orientation. In addition, provide evidence that both amyloid-beta fragments directly inhibit channel open probability hBK channel. This provides high-throughput system targeting data-intensive technique studying gating mechanisms.