Analysis of interaction between liposome membranes induced by stress condition: utilization of liposomes immobilized on indium tin oxide electrode.

作者: Haruyuki Ishii , Toshinori Shimanouchi , Hiroshi Umakoshi , Ryoichi Kuboi

DOI: 10.1016/J.JBIOSC.2009.05.009

关键词: ChromatographyPolymerStress conditionsFluorescenceIndium tin oxideChemistryMembraneChemical engineeringLiposomeFluorescence microscopeElectrode

摘要: Abstract NBD-cholesterol (NBD-Ch)-modified liposome was immobilized on indium tin oxide (ITO) electrode via the covalent binding method. The transfer of NBD-Ch between liposomes and target observed by using a fluorescent microscope. addition suspension co-incubated with α-chymotrypsin or stimuli-responsive polymer to surface above ITO electrode, enhanced liposome–liposome interaction, resulting in promotion transfer. apparent rate constant found be correlated index for interaction evaluated an chromatography. This suggests that present method liposome-immobilized effective evaluate induced protein under stress conditions.

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