Detection of a heat stress-mediated interaction between protein and phospholipid membrane using dielectric measurement.

摘要: The dielectric response of lipid bilayer membrane vesicles (liposomes) prepared using either phosphatidylcholine from egg (EPC) or 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) was analyzed at a frequency range 0.1 to 100 MHz. A marked dispersion for EPC and POPC liposome suspensions observed above 1 An appropriate analysis the curve performed Cole-Cole equation Debye found provide method determination parameters. Among parameters, characteristic second around 50 MHz varied corresponding with changes in test conditions. Of particular note is that an anomalous change presence protein corresponded degree hydrophobic interaction between proteins liposomes. value MHz, as well decrease permittivity over tested, are indicators