Cytotoxicity and fluorescence visualization of ergot alkaloids in human cell lines.

作者: Dennis Mulac , Silke Lepski , Franziska Ebert , Tanja Schwerdtle , Hans-Ulrich Humpf

DOI: 10.1021/JF304569Q

关键词: HT29 CellsApoptosisCytotoxicityCytotoxic T cellBiological activityAlkaloidReceptorStereochemistryBiologyBiochemistryCell culture

摘要: The ergot alkaloids as secondary metabolites from fungi of the genus Claviceps are focus many investigations because their pharmacological and toxicological properties. main effects referred to an interaction with several receptor systems in human body. It is well-known that able isomerize one isomer being biologically active only weakly active, whereas activity restricted interactions. Latest have proven also show cytotoxic induce apoptosis primary cells. These seem correlate accumulation was aim our current study determine such cancer cell lines, derivatives used tumor therapy. Our results confirm apoptotic two lines (HepG2 HT-29) a high range, measurements interesting correlation between alkaloid concentrati...

参考文章(36)
B. T. Larson, M. D. Samford, J. M. Camden, E. L. Piper, M. S. Kerley, J. A. Paterson, J. T. Turner, Ergovaline Binding and Activation of D2 Dopamine Receptors in GH4ZR7 Cells Journal of Animal Science. ,vol. 73, pp. 1396- 1400 ,(1995) , 10.2527/1995.7351396X
G. Maurer, W. Frick, Elucidation of the structure and receptor binding studies of the major primary, metabolite of dihydroergotamine in man. European Journal of Clinical Pharmacology. ,vol. 26, pp. 463- 470 ,(1984) , 10.1007/BF00542142
S Yoshitomi, K Ikemoto, J Takahashi, H Miki, M Namba, S Asahi, Establishment of the transformants expressing human cytochrome P450 subtypes in HepG2, and their applications on drug metabolism and toxicology. Toxicology in Vitro. ,vol. 15, pp. 245- 256 ,(2001) , 10.1016/S0887-2333(01)00011-X
J. M. Duringer, R. Lewis, L. Kuehn, T. Fleischmann, A. M. Craig, Growth and hepatic in vitro metabolism of ergotamine in mice divergently selected for response to endophyte toxicity. Xenobiotica. ,vol. 35, pp. 531- 548 ,(2005) , 10.1080/00498250500153838
Maria Weidner, Tanja Welsch, Florian Hübner, Gerald Schwerdt, Michael Gekle, Hans-Ulrich Humpf, Identification and apoptotic potential of T-2 toxin metabolites in human cells. Journal of Agricultural and Food Chemistry. ,vol. 60, pp. 5676- 5684 ,(2012) , 10.1021/JF300634K
Carolin Franzmann, Johannes Wächter, Natascha Dittmer, Hans-Ulrich Humpf, Ricinoleic Acid as a Marker for Ergot Impurities in Rye and Rye Products Journal of Agricultural and Food Chemistry. ,vol. 58, pp. 4223- 4229 ,(2010) , 10.1021/JF1006903
I. Vermes, C. Haanen, D J. Richel, M.R. Schaafsma, E. Kalsbeek-Batenburg, C.P.M. Reutelingsperger, Apoptosis and secondary necrosis of lymphocytes in culture. Acta Haematologica. ,vol. 98, pp. 8- 13 ,(1997) , 10.1159/000203546