Evidence for Two Different Regulatory Mechanisms Linking Replication and Segregation of Vibrio cholerae Chromosome II
作者： Tatiana Venkova-Canova , Jong Hwan Baek , Peter C. FitzGerald , Melanie Blokesch , Dhruba K. Chattoraj
DOI: 10.1371/JOURNAL.PGEN.1003579
关键词: Control of chromosome duplication 、 Origin recognition complex 、 Replication factor C 、 DnaA 、 Pre-replication complex 、 Biology 、 DNA replication factor CDT1 、 Ter protein 、 Genetics 、 SeqA protein domain 、 Genetics(clinical) 、 Cancer research 、 Ecology, Evolution, Behavior and Systematics 、 Molecular biology
摘要: Understanding the mechanisms that coordinate replication initiation with subsequent segregation of chromosomes is an important biological problem. Here we report two replication-control mediated by a chromosome protein, ParB2, encoded II model multichromosome bacterium, Vibrio cholerae. We find ChIP-chip assay centromere binding spreads beyond and covers inhibitory site (a 39-mer). Unexpectedly, without nucleation at centromere, ParB2 could also bind directly to related 39-mer. The 39-mers are strongest inhibitors they mediate inhibition initiator protein. thus appears promote out-competing using mechanisms: spreading into one direct other. suggest both these novel chromosomes.
paperity.org参考文章(55)
Michael A. Fogel, Matthew K. Waldor, Distinct segregation dynamics of the two Vibrio cholerae chromosomes Molecular Microbiology. ,vol. 55, pp. 125- 136 ,(2004) , 10.1111/J.1365-2958.2004.04379.X
Nilangshu Das, Dhruba K. Chattoraj, Origin pairing (‘handcuffing’) and unpairing in the control of P1 plasmid replication Molecular Microbiology. ,vol. 54, pp. 836- 849 ,(2004) , 10.1111/J.1365-2958.2004.04322.X
Minsang Shin, Arvin Cesar Lagda, Jae Woong Lee, Abhay Bhat, Joon Haeng Rhee, Jeong-Sun Kim, Kunio Takeyasu, Hyon E. Choy, Gene silencing by H-NS from distal DNA site Molecular Microbiology. ,vol. 86, pp. 707- 719 ,(2012) , 10.1111/MMI.12012
Kenn Gerdes, Jakob Møller-Jensen, Rasmus Bugge Jensen, Plasmid and chromosome partitioning: surprises from phylogeny. Molecular Microbiology. ,vol. 37, pp. 455- 466 ,(2002) , 10.1046/J.1365-2958.2000.01975.X
Therese G. Brendler, Ann L. Abeles, Lucretia D. Reaves, Stuart J. Austin, The iteron bases and spacers of the P1 replication origin contain information that specifies the formation of a complex structure involved in initiation. Molecular Microbiology. ,vol. 23, pp. 559- 567 ,(1997) , 10.1046/J.1365-2958.1997.D01-1869.X
Nora L. Sullivan, Kathleen A. Marquis, David Z. Rudner, Recruitment of SMC by ParB-parS Organizes the Origin Region and Promotes Efficient Chromosome Segregation Cell. ,vol. 137, pp. 697- 707 ,(2009) , 10.1016/J.CELL.2009.04.044
Katherine P Lemon, Alan D Grossman, Movement of replicating DNA through a stationary replisome. Molecular Cell. ,vol. 6, pp. 1321- 1330 ,(2000) , 10.1016/S1097-2765(00)00130-1
Ci Ji Lim, Yixun R. Whang, Linda J. Kenney, Jie Yan, Gene silencing H-NS paralogue StpA forms a rigid protein filament along DNA that blocks DNA accessibility Nucleic Acids Research. ,vol. 40, pp. 3316- 3328 ,(2012) , 10.1093/NAR/GKR1247
R. Kadoya, J. H. Baek, A. Sarker, D. K. Chattoraj, Participation of chromosome segregation protein ParAI of Vibrio cholerae in chromosome replication. Journal of Bacteriology. ,vol. 193, pp. 1504- 1514 ,(2011) , 10.1128/JB.01067-10
Heath Murray, Jeff Errington, Dynamic Control of the DNA Replication Initiation Protein DnaA by Soj/ParA Cell. ,vol. 135, pp. 74- 84 ,(2008) , 10.1016/J.CELL.2008.07.044