Establishing correlations in the en-mass migration of dermal fibroblasts on oriented fibrillar scaffolds

摘要: Abstract Wound healing proceeds via fibroblast migration along three dimensional fibrillar substrates with multiple angles between fibers. We have developed a technique for preparation of scaffolds where the fiber diameters and adjacent layers could be precisely controlled. Using agarose droplet method we were able to make accurate determinations dependence speed, focal adhesion distribution, nuclear deformation on diameter, spacing, angle layers. found that oriented single layers, whose exceeded 1 μm, large complexes formed in linear arrangement axis cell motion was highly correlated. On multi layered most sites reformed at junction points speed determined by which followed parabolic function minimum 30°. these surfaces observed 25% increase number similar decrease degree deformation, both phenomena associated decreased mobility. These results underscore importance substrate morphology en-mass dynamics. Statement Significance En-mass is an essential component wound process can determine rate scar formation. Yet, publications this topic focused functions. Here describe new apparatus designed well controlled geometries. show fibroblasts undergoing topography. Significant differences adhesions exist cells migrating flat versus fibers, distance