作者: Etsushi Kuroda , Tsutomu Sugiura , Kazumasa Okada , Kazuya Zeki , Uki Yamashita
DOI: 10.4049/JIMMUNOL.166.3.1650
关键词: Biochemistry 、 Spleen 、 Immune system 、 CXCL16 、 Cell biology 、 CD40 、 Chemokine 、 CCR4 、 Chemistry 、 CXC chemokine receptors 、 Prostaglandin E2
摘要: PGE(2) has been known to suppress Th1 responses. We studied the role of in two representative chemokines, macrophage-derived chemokine (MDC) and IFN-inducible protein-10, production by LPS- or CD40-stimulated spleen cells. The MDC, one ligands for CCR4 preferentially expressed on Th2, was enhanced nonstimulated, LPS-, CD40-, CD3-stimulated cells pretreatment with PGE(2), while a ligand CXC receptor 3 Th1, suppressed. MDC also IL-4, IL-5, intracellular cAMP-elevating agents such as dibutyryl cAMP 3-isobutyl-1-methylxanthine, effect additive. However, IL-6, IL-10, TGF-beta, neutralization IFN-gamma IL-12 had no production. B cells, macrophages, dendritic were main producers T produced only small amount MDC. equally stimulated LPS anti-CD40 Ab, that macrophages more markedly further these These results indicate regulates Th1/Th2-related this is new function regulation Th2 immune responses at induction activation stages.