Signal amplification with lollipop probes
作者： Patricia Bray-Ward , David C. Ward , Gyanendra Kumar , Michael J. Lane
DOI:
关键词: Nucleic acid 、 Signal amplification 、 Stereochemistry 、 Oligomer 、 Chemistry 、 Sequence (medicine) 、 Biochemistry
摘要: Disclosed is a method and compositions for the sensitive detection of amount location specific nucleic acid sequences. The makes use branched oligomer, referred to as lollipop that has tail portion, right arm left portion. These three components are joined at common junction making three-tailed structure. two arms each end with sequences complementary adjacent in target sequence. This allows be ligated together when oligomer hybridized sequence, thus topologically linking portion can then detected
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