Gene expression of the taurine transporter and taurine biosynthetic enzymes in rat kidney after antidiuresis and salt loading.
作者： Marc Bitoun , Olivier Levillain , Marcel Tappaz
关键词: Transporter 、 Biochemistry 、 Taurine 、 Gene expression 、 Chemistry 、 Betaine transporter 、 Cysteine dioxygenase 、 Osmolyte 、 Diuresis 、 Kidney
摘要: Taurine is thought to be an osmolyte in the kidney medulla. We have investigated gene expression of taurine transporter (TauT) and enzymes biosynthesis, cysteine dioxygenase (CDO) sulfinate decarboxylase (CSD). achieved this by measuring their mRNA levels using reverse transcriptase polymerase chain reaction (RT-PCR) five regions rats various hydration states; namely, normal hydration, after 2 days antidiuresis following chronic diuresis finally acute salt loading. The well-established tonicity-sensitive genes coding for aldose reductase (AR), sodium myo-inositol (SMIT) betaine (BGT1) were also determined sake comparison. In normally hydrated rats, TauT-, CDO-, CSD-mRNA enriched outer stripe medulla (OS). Following antidiuresis, TauT, CDO, CSD, SMIT, BGT1 AR all similarly increased papilla when compared with submitted a diuresis. After loading, level like that SMIT BGT1, was overexpressed OS whereas CDO CSD remained unchanged. Like genes, appear which can activated vivo hypertonicity rat kidney. However, tonicity-induced activation TauT more sensitive than genes.
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