Proteinases in Excretory-Secretory Products of Toxocara canis Second-Stage Larvae: Zymography and Modeling Insights

摘要: Components released in excretory-secretory products of Toxocara canis larvae (TES) include phosphatidylethanolamine-binding proteins (TES26), mucins (TES120, MUC2-5), and C-type lectins (TES32, TES70) their biochemical, immunological, diagnostic properties have been extensively studied albeit proteinase activities towards physiological substrates are almost unknown. Proteolytic TES samples were first analyzed by gel electrophoresis with gelatin as substrate. Major ~400, 120, 32 kDa relatively similar over a broad pH range (5.5–9.0) all these the serine-type leupeptin abolished gelatinolysis. Further, ~400 kDa component degraded tested (laminin, fibronectin, albumin, goat IgG) 120 kDa albumin IgG while proteinases lower MW (45, 32, 26 kDa) only laminin preferentially at alkaline (9.0). By protein modeling approaches using known sequences components, TES26 MUC4 displayed folding patterns significantly related to reference serine proteinases. These data suggest that most secreted vitro infective T. intriguing nature but otherwise help parasite affect multiple components somatic organs bodily fluids within infected host.