Cytokine production and signalling in human THP-1 macrophages is dependent on Toxocara canis glycans

摘要: The effect of Toxocara canis antigens on cytokine production by human THP-1 macrophages was studied in vitro. Excretory-Secretory products (TES) and recombinant mucins (Tc-MUC-2, Tc-MUC-3, Tc-MUC-4, Tc-MUC-5) as well deglycosylated forms these were used the study. TES stimulated to produce innate proinflammatory IL-1β, IL-6, TNF-α cytokines regardless presence glycans. Recombinant induced glycan-dependent production. Sugar moieties led at least 3-fold higher regulatory IL-10 cytokines. glycans also affected downstream signalling pathways cells. most prominent difference noted AKT AMPK kinase activation. phosphorylation observed cells with glycosylated mucins, whereas treatment phosphorylation. MAP family members such JNK p38 c-Jun transcription factor phosphorylated both cases what suggests that toll-like receptor may be involved mucin-treated macrophages. This pathway is however modified other molecules only containing intact sugars significantly