Toxocara canis: Proteinases in perivitelline fluid from hatching eggs

摘要: Developing larvae of Toxocara canis may secrete several kinds enzymes within the egg perivitelline fluid (EPF) prior to and during hatching. In particular, proteinases in EPF could play a role larval emergence host gastrointestinal lumen but its presence nature is unknown. this work, proteolytic activities hatching T. were identified analysed by substrate gel electrophoresis at different pH values using type specific protease inhibitors. Three bands 91, 68 38 kDa showed gelatinolytic activity all proteinase from was aspartic-type since it inhibited pepstatin A. Interestingly, significantly higher observed acidic (< or =5.5). These data suggest that developmentally secretes accumulates aspartic with pH-dependent might help parasite take advantage conditions microenvironment where induced executed.