A simple quantitative assay for ristocetin cofactor activity using microtiter plate and ELISA plate reader
作者： Suksham Puri , Cheryl Gresham , K.J. Kao
DOI: 10.1016/0049-3848(94)90116-3
关键词: Von Willebrand disease 、 Ristocetin 、 Standard curve 、 Quantitative analysis (chemistry) 、 Platelet 、 Biochemistry 、 Agglutination (biology) 、 Plate reader 、 Microtiter plate 、 Chemistry 、 Hematology
摘要: Ristocetin cofactor activity is the test of choice for diagnosing von Willebrand disease. There however no simple and precise quantitative method its rapid determination in clinical laboratory. We describe here a new approach measuring ristocetin using an ELISA plate reader. Paraformaldehyde-fixed platelets, diluted plasma samples were mixed incubated wells microtiter plate. Platelet agglutination was measured by light absorbance at 405 nm. Measurements platelet 1x to 64x dilution normal pooled used construct standard curve. Further characterization showed significant linear correlation between results determined aggregometer assay (p < 0.0001), that intra- inter-assay variations 6% 17%, respectively. Therefore, newly developed provides way quantitation activity.
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sci-hub.se 参考文章(16)
F Rodeghiero, G Castaman, E Dini, Epidemiological Investigation of the Prevalence of von Willebrand's Disease Blood. ,vol. 69, pp. 454- 459 ,(1987) , 10.1182/BLOOD.V69.2.454.454
John D. Olson, William J. Brockway, David N. Fass, Mark A. Magnuson, E. J. Walter Bowie, Evaluation of ristocetin-Willebrand factor assay and ristocetin-induced platelet aggregation. American Journal of Clinical Pathology. ,vol. 63, pp. 210- 218 ,(1975) , 10.1093/AJCP/63.2.210
Margaret A. Howard, B. G Firkin, Ristocetin--a new tool in the investigation of platelet aggregation. Thrombosis and Haemostasis. ,vol. 26, pp. 362- 369 ,(1971) , 10.1055/S-0038-1653684
Francesco Rodeghiero, Giancarlo Castaman, Alberto Tosetto, von Willebrand factor antigen is less sensitive than ristocetin cofactor for the diagnosis of type I von Willebrand disease--results based on an epidemiological investigation. Thrombosis and Haemostasis. ,vol. 64, pp. 349- 352 ,(1990) , 10.1055/S-0038-1647316
K.J. Kao, S.V. Pizzo, P.A. McKee, Factor VIII/von Willebrand protein. Modification of its carbohydrate causes reduced binding to platelets. Journal of Biological Chemistry. ,vol. 255, pp. 10134- 10139 ,(1980) , 10.1016/S0021-9258(19)70438-4
K.M. Brinkhous, James E. Graham, H.A. Cooper, J.P. Allain, R.H. Wagner, Assay of von willebrand factor in von willebrand's disease and hemophilia: Use of a macroscopic platelet aggregation test Thrombosis Research. ,vol. 6, pp. 267- 272 ,(1975) , 10.1016/0049-3848(75)90074-2
John G. Kelton, Jean Bishop, Cedric J. Carter, Jack Hirsh, A comparison of the quantitative ristocetin von Willebrand factor assay by using fresh and fixed platelets. Thrombosis Research. ,vol. 18, pp. 477- 483 ,(1980) , 10.1016/0049-3848(80)90343-6
Eric J. Werner, Thomas C. Abshire, Donna S. Giroux, Eathyl L. Tucker, Emmett H. Broxson, Relative value of diagnostic studies for von Willebrand disease The Journal of Pediatrics. ,vol. 121, pp. 34- 38 ,(1992) , 10.1016/S0022-3476(05)82537-5
A. Zaleski, J.D. Olson, A rapid assay for von Willebrand factor Thrombosis Research. ,vol. 51, pp. 462- 468 ,(1988) , 10.1016/0049-3848(88)90383-0
Harvey J. Weiss, Leon W. Hoyer, Frederick R. Rickles, André Varma, John Rogers, Quantitative Assay of a Plasma Factor Deficient in von Willebrand's Disease that is Necessary for Platelet Aggregation. RELATIONSHIP TO FACTOR VIII PROCOAGULANT ACTIVITY AND ANTIGEN CONTENT Journal of Clinical Investigation. ,vol. 52, pp. 2708- 2716 ,(1973) , 10.1172/JCI107465