Preparation and selection of sperm for IVF and ICSI

作者: Charles L. Bormann , Jose R. Alagretti , Eduardo L. A. da Motta , Paulo Serafini , Gary D. Smith

DOI: 10.1007/978-1-4419-1436-1_38

关键词: AndrologySpermSpermatozoonAbnormal sperm morphologyHuman fertilizationSperm washingSperm motilityBiologyIn vitro fertilisationIntracytoplasmic sperm injection

摘要: Approximately 50% of all infertility problems are due to male factors. Some the factors preventing natural insemination or fertilization include ejaculatory problems, low sperm counts, abnormal morphology, diminished motility and function, and, often times, a combination several these In vitro (IVF) was originally developed as tool overcome tubal defects in females has since been widely utilized achieve from men with variety parameters. cases which quality quantity severely compromised, injection spermatozoon into ooplasm mature oocyte is possible referred intracytoplasmic (ICSI). Only single required perform ICSI, yet processing, isolation, selection healthy subfertile man an environment can be quite challenging. The objective this chapter discuss most commonly used methods separation also highlight new currently being tested for IVF ICSI application. addition, describes process best suits factor question advantages disadvantages associated each method. Finally, stringent morphological criteria necessary select ICSI.

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