Hyaluronic acid (Sperm Select) improves retention of sperm motility and velocity in normospermic and oligospermic specimens.

摘要: The effects of Sperm Select (Pharmacia AB, Uppsala, Sweden), a hyaluronic acid medium, on the motility and membrane integrity properties sperm were studied. In 15 normospermic specimens after overnight incubation, parameters in control versus group as follows (mean±SEM): motility, 18.8%±2.8% 27.4%±2.9%; velocity, 21.5±2.4 27.2±2.2 μ m/s; linearity, 3.8±0.3 4.4±0.2; lateral head displacement, 1.5±0.2 1.9±0.1 m; tail beat/cross frequency, 8.8±1.3 10.8±1.4Hz. density motile was 10.8±2.3 18.5±2.5×10 6 sperm/mL. Finally, velocity coefficient, multiple linear 4.6±1.1 8.1±1.4. However, we found no related differences when testing with hypoosmotic swelling supravital staining. Thus, improves retention (most prominently velocity) apparently due to direct action metabolism or contractility rather than preservation integrity. 20 Oligospermic specimens, caused similar improvements duration could be predicted from degree enhancement short-term exposure. A modified protocol is described that further increases yield without centrifugation step.