Aberrations of the classic codon reading scheme during protein synthesis in vitro.

摘要: Using a protein synthesizing in vitro system programmed with MS2-RNA, the ability of alanine tRNAs anticodons U*GC (U* represents 5-oxyacetic acid uridine monophosphate) and IGC to read codons coat cistron MS2 has been determined both under conditions no competition, where alanyl-tRNA used was only aminoacylated tRNAAla present system, experiments two alanyl-tRNAs were competing against each other. Under can all four codons. However, when compete for codon GCC, anticodon IGC, which three positions according rules Watson-Crick base pairing, is considerably more efficient than U*GC, misreads by reading first presumably disregards third nucleotide codon. The outcome competition also reveals apparent violations wobble restrictions: reads GUU almost as effectively does effective GCG.