Generation of shear adhesion map using SynVivo synthetic microvascular networks.

摘要: Cell/particle adhesion assays are critical to understanding the biochemical interactions involved in disease pathophysiology and have important applications quest for development of novel therapeutics. Assays using static conditions fail capture dependence on shear, limiting their correlation with vivo environment. Parallel plate flow chambers that quantify under physiological fluid need multiple experiments generation a shear map. In addition, they do not represent scale morphology require large volumes (~ml) reagents experiments. this study, we demonstrate map from single experiment microvascular network based microfluidic device, SynVivo-SMN. This device recreates complex vasculature including geometric scale, morphological elements, features cellular an vitro format, thereby providing biologically realistic environment basic applied research behavior, drug delivery, discovery. The assay was demonstrated by studying interaction 2 µm biotin-coated particles avidin-coated surfaces microchip. entire range observed microvasculature is obtained enabling vs. conditions.