Strain‐Dependent Variations in the Response of Cancellous Bone to Ovariectomy in Mice

摘要: The goal of this study was to characterize the skeletal response ovariectomy in mice (129P3, C57BL/6, and B6129PF2) commonly used gene manipulation studies evaluate their potential as preclinical models postmenopausal osteoporosis. magnitude cancellous bone loss cellular indices increased turnover varied with mouse type site, but general, were less pronounced consistent than Sprague-Dawley rats, established model for loss. Introduction: ovariectomized (OVX) rat is most widely rodent However, underlying mechanisms disorders, including osteoporosis, have been explored predominantly mouse. purpose (129P3 C57BL/6 inbred strains F2 hybrid offspring, B6129PF2), knockout overexpression studies, loss. Materials Methods: OVX or sham-operated at 4 months age killed 1 3 after surgery. Lumbar vertebrae distal femora subjected histomorphometric assessment. Results: Mice two hybrids (will be referred strain remainder abstract) lost vertebral OVX; volume (BV/TV) 20% 27% lower surgery, respectively. decreased BV/TV associated an increase osteoclast surface month 129P3 only. Osteoblast by both irrespective strain. formation rate not altered any strains. In contrast lumbar vertebrae, differed femur among three had no significant effect on B6129PF2 strain, reduced surgery whereas Osteoclast affected either time-point 116% strains. Conclusions: rat. Although will continue provide insights into genetic influences mass turnover, caution should exercised when using mice, hybrids, testing therapies