Immunocytochemical localization of cytoskeletal proteins and electron microscopy of detergent extracted tachyzoites of Toxoplasma gondii.

摘要: Actin was located in tachyzoites of Toxoplasma gondii by indirect immunofluorescence using anti-actin antibodies. Fluorescence seen the region conoid almost all cells. In about 75% cells, whole anterior showed fluorescence and 25% cells posterior region. No when permeabilized parasites were incubated presence NBD-phallacidin which binds to F-actin. This observation, associated fact that no microfilaments transmission electron micrographs Triton X-100 extracted, tannic acid-glutaraldehyde (TA-GA) fixed indicate T. actin is predominantly monomeric form (G-actin). observed antibodies specific desmin, vimentin keratin. Examination TA-GA outer membrane partially removed while inner complex not, but had a corrugated aspect. Connections between sub-pellicular microtubules latter seen.