Relationships among cytotoxicity, lysosomal breakdown, chromosome aberrations, and DNA double-strand breaks.

摘要: Abstract Certain chemicals that are either weak or non-carcinogens had been previously shown to induce DNA single-strand breaks in rat hepatocytes, but only at cytotoxic doses. In contrast, stronger carcinogens induced non-toxic This report shows the strong and mutagens cadmium sulfate, sodium dichromate, dimethyl N -methyl- ′-nitro- -nitrosoguanidine all concentrations, they also double-strand concentrations closely correlated with cytotoxicity. Some produced single- breaks, acutely concentrations. We suggest result from a cell-mediated process such as release of DNAase lysosomes other cellular compartments, might occur during response toxic damage. Experiments -dodecyl imidazole (NDI), lysosomal detergent, show breakdown alone is inducer DSBs, combination prior chemical damage by MNNG synergistically induces DSBs BHK cells. -Dodecyl chromosomal aberrations CHO cells which cause cytotoxicity, cell cycle delay, breakdown. These results toxicity leads limited aberrations. Cells have sublethally damaged can repair these damages survive could become transformed DNA-damaging mechanisms associated carcinogenesis.