Decellularized hASCs-derived matrices as biomaterials for 3D in vitro approaches.
作者： Fátima Raquel Maia , Rui Luís Reis , Joaquim Miguel Oliveira , None
DOI: 10.1016/BS.MCB.2019.11.019
关键词: Decellularization 、 Regenerative medicine 、 In vitro 、 Computational biology 、 Biology 、 Biomaterial 、 Extracellular matrix
摘要: Abstract Tissue regeneration strategies have been greatly evolving in the last years due to use of more realistic approaches. These approaches rely biomaterials for development three dimensional (3D) structures that emulate vivo microenvironment different tissues. Recently, extracellular matrices (ECM) secreted by cells caught a great deal attention as an attractive biomaterial 3D structures. In fact, and/or cellular culture conditions gave rise ECM's compositions, which can be used physiologically relevant Nevertheless, recovery cell-derived ECM requires proper decellularization protocol. Herein, we report protocol recover produced human adipose derived stem cells. This comprises multiple steps (chemical, physical or enzymatic) are described here detail. Furthermore, it is describe methods evaluate effectiveness this Overall, enables production hASCs-derived further vitro models tissue strategies.
sci-hub.se 参考文章(23)
Takashi Hoshiba, Guoping Chen, Chiho Endo, Hiroka Maruyama, Miyuki Wakui, Eri Nemoto, Naoki Kawazoe, Masaru Tanaka, Decellularized Extracellular Matrix as an In Vitro Model to Study the Comprehensive Roles of the ECM in Stem Cell Differentiation Stem Cells International. ,vol. 2016, pp. 6397820- 6397820 ,(2016) , 10.1155/2016/6397820
V.M. Correlo, J.M. Oliveira, J.F. Mano, N.M. Neves, R.L. Reis, Natural Origin Materials for Bone Tissue Engineering – Properties, Processing, and Performance Principles of Regenerative Medicine (Second edition). pp. 557- 586 ,(2011) , 10.1016/B978-0-12-381422-7.10032-X
Qi Xing, Keegan Yates, Mitchell Tahtinen, Emily Shearier, Zichen Qian, Feng Zhao, Decellularization of fibroblast cell sheets for natural extracellular matrix scaffold preparation. Tissue Engineering Part C-methods. ,vol. 21, pp. 77- 87 ,(2015) , 10.1089/TEN.TEC.2013.0666
Lindsay E. Fitzpatrick, Todd C. McDevitt, Cell-derived matrices for tissue engineering and regenerative medicine applications. Biomaterials Science. ,vol. 3, pp. 12- 24 ,(2015) , 10.1039/C4BM00246F
Takashi Hoshiba, Naoki Kawazoe, Guoping Chen, The balance of osteogenic and adipogenic differentiation in human mesenchymal stem cells by matrices that mimic stepwise tissue development Biomaterials. ,vol. 33, pp. 2025- 2031 ,(2012) , 10.1016/J.BIOMATERIALS.2011.11.061
Paul E. Bourgine, Benjamin E. Pippenger, Atanas Todorov, Laurent Tchang, Ivan Martin, Tissue decellularization by activation of programmed cell death. Biomaterials. ,vol. 34, pp. 6099- 6108 ,(2013) , 10.1016/J.BIOMATERIALS.2013.04.058
Peter M. Crapo, Thomas W. Gilbert, Stephen F. Badylak, An overview of tissue and whole organ decellularization processes Biomaterials. ,vol. 32, pp. 3233- 3243 ,(2011) , 10.1016/J.BIOMATERIALS.2011.01.057
S. Tamir Rashid, Jonathan D. Humphries, Adam Byron, Ameet Dhar, Janet A. Askari, Julian N. Selley, David Knight, Robert D. Goldin, Mark Thursz, Martin J. Humphries, Proteomic analysis of extracellular matrix from the hepatic stellate cell line LX-2 identifies CYR61 and Wnt-5a as novel constituents of fibrotic liver. Journal of Proteome Research. ,vol. 11, pp. 4052- 4064 ,(2012) , 10.1021/PR3000927
Y. Higuchi, N. Shiraki, K. Yamane, Z. Qin, K. Mochitate, K. Araki, T. Senokuchi, K. Yamagata, M. Hara, K. Kume, S. Kume, Synthesized basement membranes direct the differentiation of mouse embryonic stem cells into pancreatic lineages Journal of Cell Science. ,vol. 123, pp. 2733- 2742 ,(2010) , 10.1242/JCS.066886
Sébastien Sart, Yuanwei Yan, Yan Li, Eric Lochner, Changchun Zeng, Teng Ma, Yan Li, Crosslinking of extracellular matrix scaffolds derived from pluripotent stem cell aggregates modulates neural differentiation. Acta Biomaterialia. ,vol. 30, pp. 222- 232 ,(2016) , 10.1016/J.ACTBIO.2015.11.016