Direct somatic embryogenesis and plantlet regeneration from seedling leaves of winged bean,Psophocarpus tetragonolobus (L.) DC.
作者： S. Dutta Gupta , R. Ahmed , D. N. De
DOI: 10.1007/BF01275504
关键词: Horticulture 、 Psophocarpus 、 Somatic embryogenesis 、 Plantlet 、 Cytokinin 、 Biology 、 Botany 、 Incubation 、 1-Naphthaleneacetic acid 、 Tissue culture 、 Murashige and Skoog medium
摘要: Excised seedling leaf segments of winged bean [Psophocarpus tetragonolobus (L.) DC.] underwent direct somatic embryogenesis under appropriate incubation conditions. Initiation and development the embryos occurred using a two-step culture method. The procedure involved for 28 days on MS basal medium supplemented with 0.1-0.5 mg/l NAA 1.0-2.0 BA (induction medium) before transfer to 0.1 IAA 2.0 (embryo medium). initial exposure low levels coincident high in induction was essential embryogenic induction. Maximum (43.3%) obtained 0.2 BA, at least 14 were required prior embryo medium. conversion frequency cotyledonary 53.3% upon containing ABA 7 followed by IBA BA. Following conversion, regenerated plantlets transferred soil showed normal morphological characteristics.
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sci-hub.se 参考文章(24)
W. A. Parrott, J. P. Moss, H. V. Mathews, R. E. Durham, M. A. Bailey, Tissue culture and regeneration in legumes. ICRISAT. pp. 115- 148 ,(1992)
H.M. Gregory, N. Haq, P.K. Evans, Regeneration of plantlets from leaf callus of the winged bean Psophocarpus tetragonolobus (L.) DC. Plant Science Letters. ,vol. 18, pp. 395- 400 ,(1980) , 10.1016/0304-4211(80)90104-2
Charleen M. Baker, Hazel Y. Wetzstein, Influence of auxin type and concentration on peanut somatic embryogenesis Plant Cell Tissue and Organ Culture. ,vol. 36, pp. 361- 368 ,(1994) , 10.1007/BF00046094
M.L. Christianson, D.A. Warnick, Temporal requirement for phytohormone balance in the control of organogenesis in vitro Developmental Biology. ,vol. 112, pp. 494- 497 ,(1985) , 10.1016/0012-1606(85)90423-3
Kirsten Finstad, Daniel C. W. Brown, Ken Joy, Characterization of competence during induction of somatic embryogenesis in alfalfa tissue culture Plant Cell Tissue and Organ Culture. ,vol. 34, pp. 125- 132 ,(1993) , 10.1007/BF00036092
K.S. Barna, A.K. Wakhlu, Somatic embryogenesis and plant regeneration from callus cultures of chickpea (Cicer arietinum L.). Plant Cell Reports. ,vol. 12, pp. 521- 524 ,(1993) , 10.1007/BF00236100
K. Suhasini, A.P. Sagare, K.V. Krishnamurthy, Direct somatic embryogenesis from mature embryo axes in chickpea (Cicer arietinum L.) Plant Science. ,vol. 102, pp. 189- 194 ,(1994) , 10.1016/0168-9452(94)90037-X
Rina Ahmed, S. Dutta Gupta, Deepesh N. De, Somatic embryogenesis and plant regeneration from leaf derived callus of winged bean [ Psophocarpus tetragonolobus (L.) DC.] Plant Cell Reports. ,vol. 15, pp. 531- 535 ,(1996) , 10.1007/BF00232988
K. S. Barna, A. K. Wakhlu, Direct somatic embryogenesis and plantlet regeneration from immature leaflets in chickpea In Vitro Cellular & Developmental Biology – Plant. ,vol. 31, pp. 137- 139 ,(1995) , 10.1007/BF02632008
KamalA. Malik, PraveenK. Saxena, Somatic embryogenesis and shoot regeneration from intact seedlings of Phaseolus acutifolius A., P. aureus (L.) Wilczek, P. coccineus L., and P. wrightii L. Plant Cell Reports. ,vol. 11, pp. 163- 168 ,(1992) , 10.1007/BF00232172