Effect of a finite-size pinhole on noise performance in single-, two-, and three-photon confocal fluorescence microscopy.

摘要: It is known that signal level in single-, two- and three-photon confocal fluorescence microscopy increases with the size of detector. Here we evaluate signal-to-noise signal-to-background criteria for these microscopes. We investigate effect pinhole on their ability to detect a weakly fluorescent point object presence uniformly background. Numerical results based paraxial approximation theory show optimization gives an optimal value size, which improved imaging performance. The resulting improvement noise performance, compared use large detector, greater than two-photon