N-methylformamide as a separation medium in capillary electrophoresis

摘要: The organic solvent N-methylformamide (NMF) has been used as a separation medium in capillary electrophoresis. advantageous properties of this compound are its high dielectric constant, solubilizing power and low conductivity, well amphiprotic character. It was shown that, unlike for most solvents, the electroosmotic flow is substantial. found to be possible utilize NMF without added electrolyte. Field strengths exceeding 1000 volts/cm could employed, while current maintained it thus obtain rapid analyses. Also, allowed analysis substances with solubility aqueous media. These features exemplified by carboxylic acids pharmaceuticals. Excellent reproducibility migration no sign electrical breakdown were observed, even under field strength conditions.