Transposition of gram-positive transposon Tn916 in Acholeplasma laidlawii and Mycoplasma pulmonis

摘要: Mycoplasma genetics has been limited by a lack of genetic tools such as selectable markers, methods to transfer DNA, and suitable vectors for cloning. Studies were undertaken examine the potential using streptococcal transposon Tn916 mycoplasma tool. The Escherichia coli plasmid pAM120, which contains Tn916, was transformed into Acholeplasma laidlawii pulmonis. Transposition chromosome apparently occurred an excision-insertion mechanism. This example shows that newly introduced DNA from other bacteria can be successfully expressed in should serve powerful tool study mycoplasmas.