Genetic transformation of Rhamnus fallax and hairy roots as a source of anthraquinones

摘要: Hairy roots of Rhamnus fallax Boiss. were induced using Agrobacterium rhizogenes strain A4M70GUS. The culture established on Woody plant media (WPM) showed a typical hairy root phenotype: rapid growth, reduced apical dominance and plagiotropism. Seven clones R. selected the basis their differences in colour branching. growth culture, measured through gain fresh mass, was done under 16-h photoperiod or dark. An increase anthraquinone (AQ) content obtained with yellow less branched roots, like clone 1 [16.43 mg g−1(d.m.)] 7 [14.21 g−1(d.m.)], compared other analysed transformed non-transformed tissue. This study presents first report successful transformation any species from family Rhamnaceae by A. analysis AQ production