Localisation of Antigenic Sites in Parathyroid Hormone

摘要: This chapter explores the localization of antigenic sites in parathyroid hormone. It discusses behavior fragments molecule standard radioimmunoassay and immunoradiometric type assay. By fractionating antisera, it has been shown to be possible develop assays that are specific for different parts molecule. Thus, addition multiplicity sites, system is complicated further by presence antibodies a single antiserum. With AS 201, reactivity with carboxy terminus better than amino but again complete displacement not possible. Radioactivity supernatant increases initial concentration An assay directed at terminal part hormone could developed from 190. should also increase still effective sensitivity sandwich using immunoadsorbent extract large volumes serum.