Properties, variants, and applications of the immunoradiometric assay method.

摘要: The immunoradiometric assay (IRMA) and 2-site IRMA techniques employ purified radioactive antibodies to convert soluble antigens into a directly detectable complex. Unreacted labelled antibody is discarded by reaction with solid-phase antigen (IRMA), or preliminary insolubilization of the unknown using (2-site IRMA). preparation properties antigen-immunoadsorbent, antibody, are discussed. Analysis dose-response curve problems in dose-interpolation similar IRMA, different from radioimmunoassay other competitive-binding systems. most significant variables include: exchange 2, stability reactivity washing solid-phase, non-specific interference serum proteins, paradoxical fall tube radioactivity at high dose IRMA) (the 'high-dose hook effect'). has advantages specificity (by separate directed 2 sites), sensitivity range (because low zero dose-response). Assay variants alternative labels, various preparations (including immunological 'spacer-arms'), non-immunological antigen, univalent antibodies, repeated extractions separations 2. Both have been adapted use anti-IgG as an additional 'universal' reagent, thereby avoiding necessity for specific antigen. These applied great variety will be especially appropriate automation.