C5a-induced chemiluminescence of human granulocytes and its amplification by a serum factor.
作者： F.-E. Maly , A. Kapp , Ursula Rother
DOI: 10.1016/S0171-2985(83)80021-7
关键词: Biochemistry 、 Radical 、 Catalase 、 Superoxide 、 Chemistry 、 Molecular biology 、 Stimulation 、 Lucigenin 、 Superoxide dismutase 、 Chemiluminescence 、 Hydroxyl radical
摘要: Purified human C5a elicits a fast chemiluminescence (CL) response from isolated granulocytes in the presence of Lucigenin (bis-N-methylacridinium nitrate). The reaction is inhibitable to more than 90% by superoxide dismutase (SOD) - final concentration 200 micrograms/ml -, about 60% catalase 10 mg/ml and 30% hydroxyl radical scavenger D-mannit 100 mM. Therefore O2- seems be oxygen responsible for most CL, while OH H2O2 are also involved. Addition normal pool serum cells 1-2 min before stimulation with strongly enhances effect dose time-dependent manner. existence "helper activity" amplifying C5a-induced CL postulated. This is, however, no specific C5a, since responses elicited chemotactic peptide f-met-phe or phorbol-myristate-acetate (PMA) enhanced preincubation serum. In contrast, ConA-induced not but decreased. Therefore, though unique represent general "adjuvans" on granulocytes, restricted certain stimuli.
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