A single-photon imaging system for the simultaneous quantitation of luminescent emissions from multiple samples
作者： F.E. Maly , A. Urwyler , H.P. Rolli , C.A. Dahinden , A.L. de Weck
DOI: 10.1016/0003-2697(88)90344-2
关键词: Optics 、 Linearity 、 Photon 、 Camera lens 、 Luminescence 、 Analytical chemistry 、 Image resolution 、 Detector 、 Microtiter plate 、 Chemistry 、 Luminescent Measurements 、 Biophysics 、 Cell biology 、 Biochemistry 、 Molecular biology
摘要: A combination of a two-dimensional photon detector (double-microchannel plate) with single-photon sensitivity and an optical projection system that allows space-resolved quantitation luminescent emissions from spatially extended objects is described. "luminescent image" the object focused onto accumulated over preset time stored in digital frame memory which counts areas interest can be read. In this study, consisted microtiter plate containing samples was placed below projecting lens (2.0/21 mm, 36 X 24-mm format camera lens) at distance 38.5 cm. Although geometry substantially limited collection, achieved only 10X less than obtained dedicated photon-counting luminometer. slightly diminished collection peripheral wells apparently caused by could corrected arithmetically. Both chemically generated luminescence (ATP bioluminescence) cell-derived, superoxide-dependent (with lucigenin as chemilumigenic probe) were detected excellent spatial resolution linearity response wide range.
elsevier.com
sci-hub.se 参考文章(11)
S J Klebanoff, J M Harlan, J R Gamble, M A Vadas, L H Sparks, J M Agosti, A M Waltersdorph, Stimulation of neutrophils by tumor necrosis factor. Journal of Immunology. ,vol. 136, pp. 4220- 4225 ,(1986)
Jayne A. Matthews, Armaiti Batki, Catherine Hynds, Larry J. Kricka, Enhanced chemiluminescent method for the detection of DNA dot-hybridization assays Analytical Biochemistry. ,vol. 151, pp. 205- 209 ,(1985) , 10.1016/0003-2697(85)90073-9
Anthony K. Campbell, Living light: chemiluminescence in the research and clinical laboratory Trends in Biochemical Sciences. ,vol. 11, pp. 104- 108 ,(1986) , 10.1016/0968-0004(86)90043-5
F. Hesford, M. Schmitt, S. Lazary, Rapid data acquisition from a microtiter plate fluorescence reader and applications in kinetic measurements Journal of Immunological Methods. ,vol. 100, pp. 269- 279 ,(1987) , 10.1016/0022-1759(87)90198-0
John Clark Sutherland, Bohai Lin, Denise C. Monteleone, JoAnn Mugavero, Betsy M. Sutherland, John Trunk, Electronic imaging system for direct and rapid quantitation of fluorescence from electrophoretic gels: application to ethidium bromide-stained DNA. Analytical Biochemistry. ,vol. 163, pp. 446- 457 ,(1987) , 10.1016/0003-2697(87)90247-8
Arne Lundin, Anders Thore, Analytical information obtainable by evaluation of the time course of firefly bioluminescence in the assay of ATP. Analytical Biochemistry. ,vol. 66, pp. 47- 63 ,(1975) , 10.1016/0003-2697(75)90723-X
Edgar Pick, Yona Keisari, A simple colorimetric method for the measurement of hydrogen peroxide produced by cells in culture Journal of Immunological Methods. ,vol. 38, pp. 161- 170 ,(1980) , 10.1016/0022-1759(80)90340-3
Walter Ruch, Philip H. Cooper, Marco Baggiolini, Assay of H2O2 production by macrophages and neutrophils with homovanillic acid and horse-radish peroxidase Journal of Immunological Methods. ,vol. 63, pp. 347- 357 ,(1983) , 10.1016/S0022-1759(83)80008-8
F.-E. Maly, A. Kapp, Ursula Rother, C5a-induced chemiluminescence of human granulocytes and its amplification by a serum factor. Immunobiology. ,vol. 164, pp. 90- 97 ,(1983) , 10.1016/S0171-2985(83)80021-7
Bernard M. Babior, Ruby S. Kipnes, John T. Curnutte, Biological defense mechanisms. The production by leukocytes of superoxide, a potential bactericidal agent. Journal of Clinical Investigation. ,vol. 52, pp. 741- 744 ,(1973) , 10.1172/JCI107236