Molecular and phylogenetic analysis and vaccine strain match of human influenza A(H3N2) viruses isolated in Northern Greece between 2004 and 2008.

摘要: Influenza A viruses are characterized by a unique genome structure, causing genetic instability, especially to the genes of haemagglutinin and neuraminidase. The objectives this research was molecular phylogenetic analysis influenza A(H3N2) strains that circulated in Northern Greece since 2004, particularly identification sequence variations comparison circulating with vaccine strains. Since 2004 Greece, total 216 clinical samples were positive for virus infections, which 83 (38.4%) attributed A(H3N2). Molecular HA 23 isolates showed all had at antigenic sites. Receptor binding sites conserved 2004-2005 2005-2006 whereas variation observed 2006-2007 (H195Y). Furthermore, alternative amino acids sialic acid receptor most 2004-2006 isolates. Some substitutions also neuraminidase sequences, however no effect on antigenicity viruses. Phylogenetic each year's revealed relatively low match A/Fujian/411/02 A/California/7/04 2005-2006, respectively, strain, A/Wisconsin/67/05. This year, glycosylation A/Serres/77/07-like stains. Constant surveillance yearly is great importance, so can be evaluated.