Short-term effects of calcium ions on the apoptosis and onset of mineralization of human dental pulp cells in vitro and in vivo

摘要: Calcium ions (Ca2+) are a major constituent of most pulp-capping materials and have an important role in the mineralization human dental pulp cells (hDPCs). A previous study by our group has shown that increased levels Ca2+ can promote hDPC-mediated long-term cultures (21 days). However, initiation occurs early stage osteogenic inductive culture, effects on hDPCs short-term (five days) not been studied detail. Furthermore, underlying mechanism which stimulates remained controversial. strong correlation between cell apoptosis and/or death identified. Thus, present hypothesized may onset through inducing their death. To verify this hypothesis, was added to growth culture medium at various concentrations. Alizarin Red S staining reverse transcription-polymerase chain reaction analysis were used evaluate mineralization. counting kit-8 fluorescein isothiocyanate-Annexin V/propidium iodide double-staining method adopted detect proliferation medium. An animal experiment scanning electron microscopic observation ceramic graft implants applied measure vivo. The results showed 5.4 9.0 mM accelerated mineralized matrix nodule formation, promoted osteopontin mRNA expression induced marked necrosis, but had no obvious effect proliferation. These findings indicated positive association timing formation as well quantity extracellular cultured hDPCs.