Effects of varied ionic calcium and phosphate on the proliferation, osteogenic differentiation and mineralization of human periodontal ligament cells in vitro

摘要: An S, Ling J, Gao Y, Xiao Y. Effects of varied ionic calcium and phosphate on the proliferation, osteogenic differentiation mineralization human periodontal ligament cells in vitro. J Periodont Res 2012; 47: 374–382. ©2011 John Wiley & Sons A/S Background Objective:  A number bone-filling materials containing (Ca2+) (P) ions have been used repair bone defects; however, effects that local release Ca2+ P has biological reactions are not fully understood. In this study, we investigated various levels (hPDLCs). Material Methods:  The hPDLCs were obtained using an explant culture method. Defined concentrations ratios to inorganic added standard induction media. ability proliferate these growth media was assayed Cell Counting Kit-8. apoptosis evaluated by fluorescein isothiocyanate–annexin V/propidium iodide double-staining Osteogenic morphological observations, alkaline phosphatase activity Alizarin Red S/von Kossa staining. mRNA expression related markers analysed RT-PCR. Results:  Within ranges ion tested, observed increased enhanced cell proliferation formation mineralized matrix nodules, whereas reduced. RT-PCR results showed elevated led a general increase Runx2 decreased expression, but gave no clear trend osteocalcin levels. Conclusion:  could significantly influence hPDLCs. range found combination 9.0 mm 4.5 mm optimal for