First Insights on the Presence of the Unfolded Protein Response in Human Spermatozoa.
作者： Santiago , Silva , Fardilha
DOI: 10.3390/IJMS20215518
关键词: HSF1 、 Motility 、 Gamete 、 Sperm 、 Cell biology 、 Hsp90 、 Somatic cell 、 Biology 、 HSP60 、 Unfolded protein response
摘要: The unfolded protein response (UPR) is involved in quality control and activated to several stressors. Although testis the UPR mechanisms are well described, their presence spermatozoa contentious. We aimed investigate of UPR-related proteins human sperm impact oxidative stress induction activation. To identify sperm, a bioinformatic approach was adopted. explore activation UPR, were exposed hydrogen peroxide (H2O2) motility, vitality, levels assessed. identified 97 showed, for first time, HSF1, GADD34, phosphorylated eIF2α. Additionally, exposure H2O2 resulted significant decrease viability motility an increase HSP90, HSP60, HSP27, eIF2α; all sensing proteins. This study gave us insight into male gamete. However, belief that devoid transcription translation highlight need clarify if these pathways same way as somatic cells.
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Meritxell Jodar, Edward Sendler, Stephen A. Krawetz, The protein and transcript profiles of human semen Cell and Tissue Research. ,vol. 363, pp. 85- 96 ,(2016) , 10.1007/S00441-015-2237-1
Xin Liu, Fu-Jun Liu, Shao-Hua Jin, Yan-Wei Wang, Xue-Xia Liu, Peng Zhu, Wen-Ting Wang, Juan Liu, Wen-Juan Wang, Comparative proteome analysis of human testis from newborn, young adult, and aged men identified spermatogenesis-associated proteins Electrophoresis. ,(2015) , 10.1002/ELPS.201500135
Moïra Rossitto, Pascal Philibert, Francis Poulat, Brigitte Boizet-Bonhoure, Molecular events and signalling pathways of male germ cell differentiation in mouse. Seminars in Cell & Developmental Biology. ,vol. 45, pp. 84- 93 ,(2015) , 10.1016/J.SEMCDB.2015.09.014
EF da Cruz e Silva, CA Fox, CC Ouimet, E Gustafson, SJ Watson, P Greengard, Differential expression of protein phosphatase 1 isoforms in mammalian brain The Journal of Neuroscience. ,vol. 15, pp. 3375- 3389 ,(1995) , 10.1523/JNEUROSCI.15-05-03375.1995
V. V. Evdokimov, K. V. Barinova, V. B. Turovetskii, V. I. Muronetz, E. V. Schmalhausen, Low Concentrations of Hydrogen Peroxide Activate the Antioxidant Defense System in Human Sperm Cells. Biochemistry. ,vol. 80, pp. 1178- 1185 ,(2015) , 10.1134/S0006297915090084
Rok Keber, Damjana Rozman, Simon Horvat, Sterols in spermatogenesis and sperm maturation. Journal of Lipid Research. ,vol. 54, pp. 20- 33 ,(2013) , 10.1194/JLR.R032326
Y. Liu, Y. Guo, N. Song, Y. Fan, K. Li, X. Teng, Q. Guo, Z. Ding, Proteomic pattern changes associated with obesity‐induced asthenozoospermia Journal of Andrology. ,vol. 3, pp. 247- 259 ,(2015) , 10.1111/ANDR.289
Mark W. Pellegrino, Amrita M. Nargund, Cole M. Haynes, Signaling the mitochondrial unfolded protein response Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. ,vol. 1833, pp. 410- 416 ,(2013) , 10.1016/J.BBAMCR.2012.02.019
Joanna Korfanty, Tomasz Stokowy, Piotr Widlak, Agnieszka Gogler-Piglowska, Luiza Handschuh, Jan Podkowiński, Natalia Vydra, Anna Naumowicz, Agnieszka Toma-Jonik, Wieslawa Widlak, Crosstalk between HSF1 and HSF2 during the heat shock response in mouse testes The International Journal of Biochemistry & Cell Biology. ,vol. 57, pp. 76- 83 ,(2014) , 10.1016/J.BIOCEL.2014.10.006
Wieslawa Widlak, Natalia Vydra, Ewa Malusecka, Volha Dudaladava, Boleslaw Winiarski, Dorota Ścieglińska, Piotr Widlak, Heat shock transcription factor 1 down-regulates spermatocyte-specific 70 kDa heat shock protein expression prior to the induction of apoptosis in mouse testes. Genes to Cells. ,vol. 12, pp. 487- 499 ,(2007) , 10.1111/J.1365-2443.2007.01069.X