Detection of HIV-1 infection in vitro using NASBA: an isothermal RNA amplification technique

摘要: Abstract Establishment of a sensitive infection assay for HIV-1 is essential successful screening antiviral agents and neutralizing antibodies. In this report, an described which measures the expression viral genomic RNA spliced mRNA intermediates in infected cells by amplification-based technique called NASBA. The extreme sensitivity method permits detection peripheral blood mononuclear (PBMC) within 48 h low dose virus. Similarly, could be detected 24 CEM HIV-1IIIB. This NASBA-based was shown to titer neutralization HIV-1111B isolate serum from human monoclonal antibody gp120. Furthermore, inhibitory effects azidothymidine (AZT) soluble CD4 on HIV-1IIIB were quantitated assay. early virus NASBA minimizes contribution secondary infection, thereby permitting more accurate evaluation may useful study phenotypically distinct isolates, differ terms their replication kinetics.