A synthetic hydrogel for the high-throughput study of cell-ECM interactions.

摘要: It remains extremely challenging to dissect the cooperative influence of multiple extracellular matrix (ECM) parameters on cell behaviour. This stems in part from a lack easily deployable strategies for combinatorial variation biochemical and biophysical properties. Here we describe simple, high-throughput platform based light-modulated hyaluronic acid hydrogels that enables imposition mutually independent spatially continuous gradients ligand density substrate stiffness. We validate this system by showing it can support mechanosensitive differentiation mesenchymal stem cells. also use show oncogenic microRNA, miR18a, is nonlinearly regulated stiffness fibronectin glioma The parallelization experiments enabled allows condensation studies would normally require hundreds single substrate. highly accessible, technique study signals experimental paradigm.