Channel-forming membrane permeabilization by an antibacterial protein, sapecin: determination of membrane-buried and oligomerization surfaces by NMR.
作者： Koh Takeuchi , Hideo Takahashi , Mariko Sugai , Hideo Iwai , Toshiyuki Kohno
关键词: Molecule 、 Protein structure 、 Surface plasmon resonance 、 Liposome 、 Biophysics 、 Phospholipid 、 Crystallography 、 Membrane 、 Vesicle 、 Chemistry 、 Peptide sequence
摘要: The action mechanism of sapecin, an antibacterial peptide with membrane permeabilization activity, was investigated. dose dependence the caused by sapecin sigmoidal, suggesting that oligomerization leads to permeabilization. Solution nuclear magnetic resonance analysis sapecin-phospholipid vesicle complex revealed surface buried in and on molecule. membrane-buried determined observing transferred cross-saturation phenomena from alkyl chains phospholipid amide protons sapecin. contains basic highly exposed hydrophobic residues, which are suitable for interacting acidic bacterial membrane. also identified comparisons between results hydrogen-deuterium exchange experiments experiments. On basis NMR we built a putative model oligomers, provides insights into insect defensins.
sci-hub.se 参考文章(23)
So LEE, Hyun MOON, Shunichiro KAWABATA, Shoichiro KURATA, Shunji NATORI, Bok LEE, A Sapecin Homologue of Holotrichia Diomphalia: Purification, Sequencing and Determination of Disulfide Pairs Biological & Pharmaceutical Bulletin. ,vol. 18, pp. 457- 459 ,(1995) , 10.1248/BPB.18.457
Takashi Kuzuhara, Yuki Nakajima, Kenji Matsuyama, Shunji Natori, Determination of the disulfide array in sapecin, an antibacterial peptide of Sarcophaga peregrina (flesh fly). Journal of Biochemistry. ,vol. 107, pp. 514- 518 ,(1990) , 10.1093/OXFORDJOURNALS.JBCHEM.A123077
Toshiyuki Kohno, Hideki Kusunoki, Kazuki Sato, Kaori Wakamatsu, A new general method for the biosynthesis of stable isotope-enriched peptides using a decahistidine-tagged ubiquitin fusion system: an application to the production of mastoparan-X uniformly enriched with 15N and 15N/13C. Journal of Biomolecular NMR. ,vol. 12, pp. 109- 121 ,(1998) , 10.1023/A:1008254603368
S Cociancich, A Ghazi, C Hetru, J.A. Hoffmann, L Letellier, Insect defensin, an inducible antibacterial peptide, forms voltage-dependent channels in Micrococcus luteus. Journal of Biological Chemistry. ,vol. 268, pp. 19239- 19245 ,(1993) , 10.1016/S0021-9258(19)36505-6
S Fujiwara, J Imai, M Fujiwara, T Yaeshima, T Kawashima, K Kobayashi, A potent antibacterial protein in royal jelly. Purification and determination of the primary structure of royalisin. Journal of Biological Chemistry. ,vol. 265, pp. 11333- 11337 ,(1990) , 10.1016/S0021-9258(19)38596-5
Ichio Shimada, Hideo Takahashi, Tamiji Nakanishi, Keiichiro Kami, Yoji Arata, A novel NMR method for determining the interfaces of large protein-protein complexes. Nature Structural & Molecular Biology. ,vol. 7, pp. 220- 223 ,(2000) , 10.1038/73331
K Matsuyama, S Natori, Purification of three antibacterial proteins from the culture medium of NIH-Sape-4, an embryonic cell line of Sarcophaga peregrina. Journal of Biological Chemistry. ,vol. 263, pp. 17112- 17116 ,(1988) , 10.1016/S0021-9258(18)37505-7
Kenji Matsuyama, Shunji Natori, Mode of action of sapecin, a novel antibacterial protein of Sarcophaga peregrina (flesh fly). Journal of Biochemistry. ,vol. 108, pp. 128- 132 ,(1990) , 10.1093/OXFORDJOURNALS.JBCHEM.A123151
Philippe BULET, Stephane COCIANCICH, Michel REULAND, Ferd SAUBER, Rainer BISCHOFF, Gilbert HEGY, Alain Van DORSSELAER, Charles HETRU, Jules A. HOFFMANN, A novel insect defensin mediates the inducible antibacterial activity in larvae of the dragonfly Aeschna cyanea (Paleoptera, Odonata). FEBS Journal. ,vol. 209, pp. 977- 984 ,(1992) , 10.1111/J.1432-1033.1992.TB17371.X
Christopher E. Dempsey, Shohta Ueno, Matthew B. Avison, Enhanced Membrane Permeabilization and Antibacterial Activity of a Disulfide-Dimerized Magainin Analogue Biochemistry. ,vol. 42, pp. 402- 409 ,(2003) , 10.1021/BI026328H