Induction of microglial reactive oxygen species production by the organochlorinated pesticide dieldrin.

摘要: Exposure to pesticides has been speculated contribute the development of sporadic Parkinson's disease (PD) characterized by a progressive degeneration nigrostriatal dopaminergic pathway. Activation brain microglia that produce various neurotoxic factors including cytokines and reactive oxygen species (ROS) increasingly associated with neurodegeneration induced toxicants. Dieldrin, highly persistent organochlorinated pesticide found enriched in substantia nigra some postmortem PD brains, shown be toxic dopamine neurons. In this study, we set out determine effect dieldrin on production ROS underlying mechanism action murine microglia. Treatment microglial cells 0.1 nM 1 microM for 24 h resulted concentration-dependent generation ROS. The dieldrin-induced was time-dependent significant observed 12-24 h, but not 6 after treatment. Furthermore, significantly reduced inhibitors NADPH oxidase, gene transcription protein synthesis. addition immortalized cells, primary microglia, astroglia. These results demonstrate nanomolar concentrations can stimulate may neurons known vulnerable oxidative damage. findings provide important information potential role relevance idiopathic PD.