Development of a melting-curve based multiplex real-time PCR assay for simultaneous detection of Streptococcus agalactiae and genes encoding resistance to macrolides and lincosamides.

摘要: Streptococcus agalactiae or Group B (GBS) remains the leading cause of infections in newborns worldwilde. Prenatal GBS screening pregnant women for vaginal-rectal colonization is recommended many countries to manage appropriate intrapartum antimicrobial prophylaxis those identified as carriers. In this study, a novel melting-curve based multiplex real-time PCR assay simultaneous detection and macrolide lincosamide resistance markers was developed. The usefulness evaluated rapid accurate prenatal screening. One hundred two who were at 35–37 weeks gestation enrolled study. analytical performance first tested using panel reference clinical bacterial fungal strains. To test performance, swabs obtained from seen teaching hospital regular care. results compared with microbiological analyses. showed 100% specificity limit 104 colony forming units equivalent per reaction. prevalence among population studied 15.7% (16/102) on positive culture results. Agreement between assays found 11 (68.75%) colonized women. Using culture-based reference, had sensitivity 91.7% (11/12, CI 59.7–99.6%), 95.5% (86/90, 89.8–98.7%), predictive value 73.3% (11/15, 44.8–91.1%) negative 98.9% (86/87, 92.9–99.9%). rapid, affordable sensitive direct swabs.