Double‐stranded RNA interferes with gene function at the single‐cell level in cereals

摘要: Double-stranded RNA (dsRNA) has been shown to specifically interfere with gene function in several organisms including tobacco and the model plant Arabidopsis. Here, we report on rapid sequence-specific interference of dsRNA cereals. Delivery cognate into single epidermal cells maize, barley or wheat by particle bombardment interfered co-bombarded UidA (GUS) TaGLP2a:GFP reporter genes. Cognate was also found endogenous genes A1 Ant18 encoding dihydroflavonol-4-reductase maize barley, respectively. Dihydroflavonol-4-reductase is an essential enzyme anthocyanin biosynthetic pathway barley. This can be induced transient expression C1- b-Peru that encode transcription factors. In presence corresponding gene, b-Peru-dependent, cell-autonomous accumulation red pigments bombarded reduced. demonstrated negatively Mlo, which encodes a negative regulator race non-specific resistance powdery mildew fungus Mlo dsRNA, transformed became more resistant, thereby phenocopying plants carry heritable loss-of mlo allele. The results suggest direct delivery cereals leads at single-cell level.