Infrared studies of octopus rhodopsin: Existence of a long-lived intermediate and the states of the carboxylic group of Asp-81 in rhodopsin and its photoproducts
作者： Satoshi Masuda , Eugene H. Monta , Mitsuo Tasumi , Tatsuo Iwasa , Motoyuki Tsuda
DOI: 10.1016/0014-5793(93)81280-D
关键词: Rhodopsin 、 Residue (chemistry) 、 Infrared spectroscopy 、 Chemistry 、 Reaction intermediate 、 Photoprotein 、 Stereochemistry 、 Photochemistry 、 Protonation 、 Side chain 、 Opsin
摘要: The infrared absorption spectra of octopus rhodopsin and its photoproducts have been observed at 282K 210K under irradiation blue orange light in a neutral condition. acid metarhodopsin-minus-rhodopsin lumirhodopsin-minus-rhodopsin difference obtained. A new intermediate (called transient metarhodopsin) with lifetime about 5 s has found to exist prior metarhodopsin. present results, together the data obtained previously, give information on state carboxylic group side chain Asp-81, which is only acidic amino-acid residue part opsin buried inside membrane. This protonated throughout transformation series, but changes going from metarhodopsin It probable that these two are different each other moiety.
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