Interaction between turkey monocytes and avian Chlamydia psittaci in the presence of Mycoplasma sp.: the importance of nitric oxide.

摘要: Abstract The interaction between Chlamydia psittaci and turkey monocytes was studied in vitro. Purified were inoculated with C. , the presence or absence of Mycoplasma hyorhinis . Whereas produced high amounts nitric oxide (NO) following inoculation M. did not induce NO production these phagocytes. strongly supported chlamydial growth, as demonstrated by inclusion forming units, positive direct immunofluorescence staining transmission electron microscopy. In contrast, upon co-inoculation a reduced replication rate observed. N G -monomethyl- L -Arginine, competitive inhibitor for enzyme NO-synthase, inhibited reversed antichlamydial activity co-inoculated monocytes. These results imply two considerations. First, chlamydiae are obligate intracellular bacteria, special care should be taken to guard cultures from mycoplasmal contamination, order prevent false when investigating response immunomodulating cells infection. Secondly, co-infection vitro has capacity inducing monocytes, through action NO, it could suggested that similar might take place vivo. Moreover, shown avian gallisepticum strains also able Considering prevalence both sp. turkeys, this interaction, pivotal role influence outcome respiratory diseases turkeys.