Development of a capillary zone electrophoresis method for rapid determination of human globin chains in α and β-thalassemia subjects

摘要: Abstract Thalassemia is an inherited autosomal recessive blood disorder characterized by the underproduction of globin chains as a consequence gene defects, resulting in malfunctioning red cells and oxygen transport. Analysis important aspect thalassemia research. In this study we developed capillary zone electrophoresis (CZE) method for human determination diagnosis hemoglobin variants. To demonstrate utility approach, α/β area ratios were determined samples from 310 patients healthy controls. The separation was performed on uncoated with simple preparation. Distinct peaks resolved 17 min, coefficients variation (CV) migration time areas ranged 0.37%–1.69% 0.46%–6.71%, respectively. Receiver operating characteristic (ROC) curve analysis gave 100% sensitivity specificity indicating β-TI/TM, 97.4% Hb H disease. Hemoglobin G-Honolulu (Hb G-Honolulu) Westmead WS) successfully detected using CZE method. This automated methodology simple, rapid cost-effective fast chains, which could be diagnostic tool field hemoglobinopathies.