Large dose means significant effect – dose and effect relationship of Chi-Dan-Tui-Huang decoction on alpha-naphthylisothiocyanate-induced cholestatic hepatitis in rats

摘要: Large dose application of traditional Chinese medicines has attracted more and attentions in recent years. However, the scientific connotation large not been clarified so far. The present study was designed to investigate protective effects Chi-Dan-Tui-Huang decoction (CDTHD) against Alpha-naphthylisothiocyanate (ANIT) induced acute cholestatic hepatitis rats explore dose-effect relationship CDTHD as a reference for clinical application. administration at series doses performed twice each day 5 days. cholestasic models were by intragastric ANIT on third administration. Then, investigated examining following parameters: body weights rats, morphological histopathological liver changes, levels serum biomarkers including alanine aminotransferase, aspartate alkaline phosphatase, total bilirubin, direct bilirubin γ-glutamyltranspeptidase. Furthermore, with correspondence analysis. In tested range doses, maximum tolerance did show any toxicity time went on. efficacy result showed that from 21.6 g/kg⋅d 86.4 g/kg⋅d exhibited significant hepatoprotective effect ANIT-induced hepatitis. It alleviated injury reversed adverse biochemical changes dose-dependent manner. Correspondence analysis Radix Paeoniae Rubra main effective component could enhance integrated is beneficial protection Especially demonstrates potent formula contributes without toxicity.