Comparative methodological analysis of erbB-2/HER-2 gene dosage, chromosomal copy number and protein overexpression in breast carcinoma tissues for diagnostic use.

摘要: Aims : This study was performed to test the validity of different methods for determining status erbB-2/HER-2 oncogene in breast cancer tissues diagnostic use. Methods and results : Forty formalin-fixed, paraffin-embedded carcinomas were investigated by fluorescence situ comparative genomic hybridization (FISH, CGH) as well immunohistochemistry (IHC) using Dako-HercepTestTM CB11 antibody (Ventana). Additionally, competitive-differential polymerase chain reaction (cdPCR) on frozen samples estimate gene dosage alterations erbB-2/HER-2. Amplification detected 12–23% protein overexpression 16–68% cases, depending methodology and/or reagent used. Perfect concordance (100%) found between results cdPCR CB11-IHC, a 97% FISH CB11-IHC. The CB11-IHC 78%; seven eight 2 + with classified nonamplified FISH. Conclusions : Our indicate that high-level expression normal can be reliably both IHC assessment paraffin material use. However, borderline results, especially those immunopositivity, should interpreted caution increased emphasis given other clinical prognostic information available.