Human Lung Cancer Cells Grown in an Ex Vivo 3D Lung Model Produce Matrix Metalloproteinases Not Produced in 2D Culture
作者： Dhruva K. Mishra , Jason H. Sakamoto , Michael J. Thrall , Brandi N. Baird , Shanda H. Blackmon
DOI: 10.1371/JOURNAL.PONE.0045308
关键词: Apoptosis 、 Ex vivo 、 Lung cancer 、 Adenocarcinoma of the lung 、 Cell culture 、 Lung 、 Matrix metalloproteinase 、 Pathology 、 Biology 、 A549 cell 、 General Biochemistry, Genetics and Molecular Biology 、 General Agricultural and Biological Sciences 、 General Medicine
摘要: We compared the growth of human lung cancer cells in an ex vivo three-dimensional (3D) model and 2D culture to determine which better mimics patients. A549 were grown 3D for 15 days. measured size formation tumor nodules counted after also stained tissue/cells Ki-67, Caspase-3. matrix metalloproteinase (MMP) levels conditioned media blood plasma from patients with adenocarcinoma lung. Organized intact vascular space formed but not culture. Proliferation apoptosis greater After days, there significantly more than model. MMP-1, MMP-9, MMP-10 production There was no MMP-9 The patient samples contained MMP-2, MMP-10. on form perfusable that grow over time. It produced MMPs seen may closely mimic biology development
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