Assessment of Proliferation and Cytotoxicity in a Biomimetic Three-Dimensional Model of Lung Cancer

摘要: Background Decellularized whole-organ scaffolds show great potential in cancer research. They have been used the biomimetic three-dimensional (3D) culture of non-small cell lung cells, allowing study unique aspects biology. However, there are no reproducible assays capable directly monitoring processes involved progression within such scaffolds. Methods The human adenocarcinoma lines H358, PC9, and SW1573 were subjected to 3D decellularized A resazurin-based reagent was perfused through scaffold determine viability over period response treatment with cisplatin or erlotinib. Results resazurin reduction perfusion assay detected a progressive increase time for all cultured scaffolds, translating into incremental populations. Also, it positive cytotoxic effect in H358- PC9-seeded after cisplatin, Moreover, identified relative resistance erlotinib H358- SW1573-seeded from this correlated histopathology, expression caspase 3, activity epidermal growth factor receptor signaling. Conclusions methods described here under conditions permit proliferation, evaluation responses therapeutic interventions, determination chemo-sensitivities.