Compensatory ion transport buffers daily protein rhythms to regulate osmotic balance and cellular physiology

摘要: Between 6-20% of the cellular proteome is under circadian control to tune cell function with cycles environmental change. For viability, and maintain volume within narrow limits, osmotic pressure exerted by changes in soluble must be compensated. The mechanisms consequences underlying compensation are not known. Here, we show cultured mammalian cells vivo that requires electroneutral active transport Na⁺, K⁺, Cl⁻ through differential activity SLC12A family cotransporters. In cardiomyocytes ex vivo, compensatory ion fluxes alter their electrical at different times day. Perturbation protein abundance has commensurate effects on composition across cycle. Thus, regulation impacts homeostasis substantial for physiology electrically such as cardiomyocytes.