The Atypical Fibrin Fibre Network in Rheumatoid Arthritis and its Relation to Autoimmunity, Inflammation and Thrombosis
作者： Johannes A. Bezuidenhout , Chantelle Venter , Timothy Roberts , Gareth Tarr , Douglas B. Kell
DOI: 10.1101/2020.05.28.121301
关键词: Rheumatoid arthritis 、 Thromboelastography 、 Coagulation 、 Fibrin 、 Inflammation 、 Thrombus 、 Thrombosis 、 Medicine 、 Pathology 、 Citrulline
摘要: Objective: The risk of cardiovascular events in patients with RA is disproportionately heightened as a result systemic inflammation. relative effect autoimmune-associated citrullination on the structure and thrombotic potential fibrin(ogen) remains unknown. We therefore compared indices vascular function, inflammation, coagulation fibrin clot composition healthy controls evaluated inter-parameter relationships. Methods. Blood samples were collected from 30 25 age- gender-matched volunteers. Levels SAA, CRP, ICAM-1 VCAM-1 was measured using sandwich immunoassay. Whole blood assessed Thromboelastography. Fibrin networks fiber investigated Scanning Electron Microscopy. detection quantification formed clots performed fluorescently labeled Citrulline monoclonal antibody Confocal Results. Concentrations CRP significantly elevated to controls. TEG parameters relating initiation (R K), rate cross-linking (α-Angle), time reach maximum thrombus generation (TMRTG) attenuated patients. Parameters strength (MA, MRTG, TGG) did not statistically differ between Logistic regression modelling revealed stronger association acute phase reactants (CRP, SAA) than endothelial function markers. Microscopic analysis denser thicker fibers [median (interquartile range) 214 (170-285) vs 120 (100-144) nm respectively, p
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