Inhibition of RhoA expression by adenovirus-mediated siRNA combined with TNF-α induced apoptosis of hepatocarcinoma cells.

摘要: Tumor necrosis factor-alpha (TNF-α) has been used as an effective treatment for Hepatocellular Carcinoma, however, inducing tumor cell apoptosis by TNF-α alone is still unsatisfactory. RhoA highly expressed in hepatocarcinoma cells and can be activated TNF-α. The activation of directly leads to a poor prognosis HCC. Therefore, we propose investigate the therapeutic effect together with siRNA. inhibition was accomplished constructing recombinant adenovirus that efficiently express siRNA HepG2 cells. AdshRNA-RhoA AdU6-control were generated adenovirus-mediated expression system. effects detected RT-PCR addition immunoblot quantify decreased levels expression, HCC demonstrated proliferation ratios significant at both mRNA protein levels: transcription 74.46%, 76.48%. rate MTT showed could strengthen suppression ability cells, resulting approximately 14.2% more than those treated only FCA TUNEL assays results revealed combined induce 52.14%-65% whereas this ratio TNF-α-alone group 21.91%-32%. Our enhance TNF-α-induced This method might useful route other tumors.